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Status |
Public on Oct 16, 2007 |
Title |
Gene expression profile of the age-related hearing loss cochlea in DBA/2J mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Age-related hearing loss (AHL) is the progressive loss of auditory function with aging. The DBA/2J (DBA) mice have been used as a model of AHL and undergoes progressive, age-related hearing loss by 12 weeks of age. Here we analyzed cochlear gene expression of 7-week-old and 36-week-old DBA mice using microarrays. Auditory brainstem response (ABR) analysis confrimed that severe age-related hearing loss occured in 36-week-old mice, whereas moderate hearing loss occured in 7-week-old mice. Comprehensive gene expression analysis identified genes correlated with AHL and revealeed that 15 mitochondrial process categories, including “mitochondrial electron transport chain”, “oxidative phosphorylation”, “respiratory chain complex I”, “respiratory chain complex IV”, and “respiratory chain complex V”, were statistically associated with AHL-correlated genes in the cochlea of 36-week-old DBA mice, and that 25 genes encoding components of the mitochondrial respiratory chain (respiratory chain complex I, IV, and V) were significantly down-regulated in the cochlea. These observations provide evidence that AHL is associated with down-regulation of genes involved in the mitochondrial respiratory chain in the cochlea of DBA mice, and suggest that mitochondrial respiratory chain dysfunction may be a key feature of AHL in mammalian cochlea. Keywords: Disease state analysis, Time course analysis
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Overall design |
To determine the effects of age-related hearing loss, each 7-week-old sample (n = 3) was compared to each 36-week-old sample (n = 3), generating a total of nine pairwise comparisons. Using DAVIS and EASE, the identified genes were assign to “GO: Biological Process” categories of Gene Ontology Consortium. Furthermore, we used EASE to determine the total number of genes that were assigned to each biological process category, and to perform Fisher exact test. Quality control measures were not used. No replicates were done. Dye swap was not used.
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Contributor(s) |
Someya S, Yamasoba T, Weindruch R, Prolla TA |
Citation(s) |
17964557 |
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Submission date |
Oct 16, 2006 |
Last update date |
Jan 08, 2019 |
Contact name |
Shinichi Someya |
E-mail(s) |
someya@wisc.edu
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Phone |
608-265-5205
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Fax |
608-262-2976
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URL |
http://www.genetics.wisc.edu/faculty/profile.php?id=140
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Organization name |
University of Wisconsin-Madison
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Department |
Medical Genetics
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Lab |
Prolla Lab
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Street address |
425 Henry Mall Dr
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City |
Madison |
State/province |
WI |
ZIP/Postal code |
53706 |
Country |
USA |
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Platforms (1) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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Samples (6)
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Relations |
BioProject |
PRJNA97781 |
Supplementary file |
Size |
Download |
File type/resource |
GSE6045_RAW.tar |
18.5 Mb |
(http)(custom) |
TAR (of CEL) |
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