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| Status |
Public on Dec 31, 2014 |
| Title |
Gene expression profiles of adult murine osteoblasts and cementoblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Cementoblasts are cells that line the surface of the tooth root and produce a mineralized tissue known as cementum. Cementum is critical for attachment of the tooth to the adjacent periodontal ligament and has a similar biochemical composition to bone, however it differs in that it lacks vasculature and innervation, and does not undergo remodelling. It has been difficult to define markers that specifically identify cementoblasts. In order to compare the transcriptome of fully developed cementoblasts with neural crest derived (calvarial) osteoblasts in vivo, we used osteocalcin-GFP (OC-GFP) mice. Cells were isolated by digestion of calvaria (osteoblasts) or extracted molars (cementoblasts) and purified by FACS sorting for GFP positive cells before RNA extraction and microarray analysis.
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| Overall design |
OC-GFP positive cells were isolated by FACS from enzymatically digested calvaria, or extracted molars from 5-7 week old OC-GFP mice. Total RNA was extracted and amplified for microarray analysis. Samples were pooled from 6-10 mice. 3 replicates are included for each sample.
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| Contributor(s) |
Matthews BG, Kalajzic I |
| Citation(s) |
26215316 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR055607 |
Mechanisms underlying commitment and differentiation of progenitor cells during bone healing |
UNIVERSITY OF CONNECTICUT HEALTH CENTER |
IVO KALAJZIC |
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| |
| Submission date |
Aug 13, 2014 |
| Last update date |
Oct 08, 2019 |
| Contact name |
Brya Matthews |
| E-mail(s) |
brya.matthews@auckland.ac.nz
|
| Organization name |
University of Auckland
|
| Department |
Molecular Medicine and Pathology
|
| Street address |
85 Park Rd
|
| City |
Grafton |
| State/province |
Auckland |
| ZIP/Postal code |
1023 |
| Country |
New Zealand |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA258167 |
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