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Series GSE60247 Query DataSets for GSE60247
Status Public on Sep 28, 2014
Title PPARγ controls alveolar macrophage identity [part1]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Tissue-resident macrophages comprise heterogeneous populations with unique functions and distinct gene expression signatures. While it has been established that they mostly originate from embryonic progenitors, the signals inducing a characteristic tissue-specific differentiation program remain unknown. Here we identify PPARγ as the crucial transcription factor determining perinatal alveolar macrophage (AM) development and identity. Development of the fetal monocyte derived AM precursor was largely abrogated in CD11c-Cre/Ppargfl/fl mice. To reveal the underlying changes in gene expression, we performed microarray analysis of sorted WT and KO AM and pre-AM from 3 different timepoints.
Part1: d2 and d11 sorts & array
Overall design pre-AM FACS-sorted from lungs of d2 and mature AM from d11 PPARgfl/fl (WT) and CD11c-cre/PPARgfl/fl (KO) mice and subsequently processed for RNA extraction and hybridization on Affymetrix microarrays. 2 biological replicates per group, each composed of pooled cells from 2 individual mice
Contributor(s) Schneider C, Nobs SP, Kurrer M, Rehrauer H, Thiele C, Kopf M
Citation(s) 25263125
Submission date Aug 08, 2014
Last update date Apr 18, 2017
Contact name Christoph Schneider
Organization name ETH Zurich
Department Department of Biology, Institute of Molecular Health Sciences
Lab Molecular Biomedicine
Street address Otto-Stern-Weg 7
City Zurich
ZIP/Postal code 8093
Country Switzerland
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (8)
GSM1468705 preAM WT d2 biological rep1
GSM1468706 preAM WT d2 biological rep2
GSM1468707 preAM KO d2 biological rep1
This SubSeries is part of SuperSeries:
GSE60249 PPARγ controls alveolar macrophage identity
BioProject PRJNA257838

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60247_RAW.tar 36.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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