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| Status |
Public on Mar 01, 2015 |
| Title |
RNA-Seq analysis to profile the transcriptome of Bone Marrow Dendritic Cells exposed to different antigen delivery systems |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we report the analysis of the transcriptome of bone marrow-derived dendritic cells exposed to the different antigen delivery systems to dissect the molecular mechanisms that orchestrate the immune response upon vaccination. RNA-Sequencing revealed that exposure of dendritic cells to diffent antigen display vehicules causes the deregulation of molecules involved in the immune response, at a different extent, depending on the carrier. In vaccinology, patterns of gene expression induced by priming antigen presenting cells (APCs), could be used to predict immunogenicity and the type of T-helper cell polarization, and/or to helpin selecting an appropriate adjuvant to administer in the vaccine formulation.
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| Overall design |
Bone marrow precursor at day 7 of GM-CSF culture were exposed or not to LPS deprived bacteriophage, bacteriophage modified to be targeted to DC or to the E2 protein scaffold
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| Contributor(s) |
De Berardinis P, D'Apice L, Trovato M, Sartorius R, Ciccodicola A, Costa V |
| Citation(s) |
25888235 |
| |
| Submission date |
Aug 08, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Piergiuseppe De Berardinis |
| E-mail(s) |
p.deberardinis@ibp.cnr.it
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| Organization name |
National Research Council
|
| Department |
Institute of Protein Biochemistry
|
| Street address |
Via P. Castellino, 111
|
| City |
Naples |
| State/province |
Italy |
| ZIP/Postal code |
80131 |
| Country |
Italy |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA257798 |
| SRA |
SRP045363 |
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