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Series GSE60019 Query DataSets for GSE60019
Status Public on Mar 24, 2015
Title Gene Expression by Mouse Inner Ear Hair Cells During Development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hair cells of the inner ear are essential for hearing and balance. As a consequence, pathogenic variants in genes specifically expressed in hair cells often cause hereditary deafness. Hair cells are few in number and not easily isolated from the adjacent supporting cells, so the biochemistry and molecular biology of hair cells can be difficult to study. To study gene expression in hair cells, we developed a protocol for hair cell isolation by FACS sorting. With nearly pure hair cells and surrounding cells, from cochlea and utricle and from embryonic day 16 to postnatal day 7, we performed a comprehensive cell-type-specific RNA-Seq study of gene expression during mouse inner ear development. Expression profiling revealed new hair-cell genes with distinct expression patterns: some are specific for vestibular hair cells, others for cochlear hair cells, and some are expressed just before or after maturation of mechanosensitivity. We found that many of the known hereditary deafness genes are much more highly expressed in hair cells than surrounding cells, suggesting that genes preferentially expressed in hair cells are good candidates for unknown deafness genes.
Overall design 3' tags of mRNA profiles of hair cells and surrounding cells from E16, P0, P4, and P7 cochlear and utricular sensory epithelia were generated by deep sequencing, using Illumina GAIIx
Web link
Contributor(s) Scheffer DI, Shen J, Corey DP, Chen Z
Citation(s) 25904789, 26209310, 25772365
Submission date Aug 01, 2014
Last update date Oct 17, 2019
Contact name David P. Corey
Phone 617 432 2507
Organization name Harvard Medical School
Department Neurobiology Department
Lab Corey Lab
Street address 220 longwood avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (18)
GSM1463871 E16Cn
GSM1463872 E16Cp
GSM1463873 E16Un
BioProject PRJNA257284
SRA SRP045182

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Supplementary file Size Download File type/resource
GSE60019_Processed_Data.txt.gz 2.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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