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Series GSE60005 Query DataSets for GSE60005
Status Public on Oct 19, 2015
Title Genome-wide maps of chromatin state in HSC, Pro B and mature B cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We have used a genome-wide ChIP-sequencing approach to define and investigate the dynamics of the cis-regulatory landscape in three developmental stages of the murine hematopoietic system. To this end, we have compared the profiles of H3K4me3, H3K4me1, H3K27ac, H3K27me3 and H3K9me2 in HSCs, committed pro-B and splenic mature B cells. We find the enhancer repertoire to be dynamically reshaped during hematopoiesis progression, surprisingly only a small fraction of primed enhancers in HSCs or committed progenitors become activated in subsequent stages. In turn, the majority of active enhancers in terminally differentiated cells are not primed in earlier stages. We also found that The heterochromatin mark H3K9me2 covers large domains that remain largerly invariant across the three stages and are depleted in both active chromatin marks and the Polycomb related mark H3K27me3.
Overall design Investigating enhancer dynamics in 3 different stages of B cell development
Contributor(s) Choukrallah M, Song S, Rolink AG, Burger L, Matthias P
Citation(s) 26477271
Submission date Aug 01, 2014
Last update date May 15, 2019
Contact name Mohamed-Amin choukrallah
Organization name FMI
Department Epigenetic
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (38)
GSM1463425 HSC_H3K4me3
GSM1463426 HSC_H3K27ac
GSM1463427 HSC_H3K4me1_rep1
BioProject PRJNA257383
SRA SRP045221

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Supplementary file Size Download File type/resource
GSE60005_RAW.tar 385.5 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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