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| Status |
Public on Oct 20, 2014 |
| Title |
RNA sequencing of monocytes and microglia from mice with experimental autoimmune encephalomyelitis illustrates a changing phenotype with disease course |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The role of microglia and infiltrating monocytes in experimental autoimmune encephalomyelitis (EAE) pathogenesis has been controversial. To gain insight into their respective roles, we developed a method for differentiating between microglia and infiltrating monocytes in the CNS using CD44. We used this system to monitor changes in cell number, activation status, and gene expression by RNA sequencing (RNA-Seq) over the course of disease. This in vivo characterization and RNA-Seq dataset improves our understanding of myeloid cell biology in the brain under inflammatory conditions and may lead to strategies to identify therapies for inflammatory pathways active in neuroinflammatory diseases.
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| Overall design |
Pooled samples from 10 mice were analyzed for both microglia and monocytes at distinct timepoints post EAE inducation. Peritoneal macrophages were isolated and analyzed for five samples, and also in a pool.
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| Contributor(s) |
Lewis ND, Hill JD, Juchem KW, Modis LK, Stefanopoulos D |
| Citation(s) |
25270668 |
| |
| Submission date |
Jul 24, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jonathan Daryl Hill |
| E-mail(s) |
jon.hill@boehringer-ingelheim.com
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| Organization name |
Boehringer Ingelheim Pharmaceuticals Inc
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| Department |
Computational Biology
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| Street address |
900 Ridgebury Road
|
| City |
Ridgefield |
| State/province |
CT |
| ZIP/Postal code |
06877 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA256045 |
| SRA |
SRP044785 |
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