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| Status |
Public on Aug 20, 2015 |
| Title |
Transcriptome imposed by Notch ligands (Jag1 or Dll4) on ckit+ and ckit- cells from E11.5 AGM |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hematopoietic Stem Cells (HSC) are originated during embryonic development from endothelial-like cells located in the ventral side of the dorsal aorta around day E10-12 of murine development. This region is called AGM for Aorta/Gonad/Mesonephros refering to the tissues around the hemogenic aorta. Cells that emerge from the endothelium and show hematopoietic traits can be distinguished by the expression of the c-kit receptor and finally acquire the CD45 marker. The effect of Notch activation by each one of the ligands has been tested on c-kit+ and ckit- cells from the endothelium of the E11.5 AGM . Incubations with stromal cell lines exposed the cells to high levels of ligand and induced changes in the transcriptome of these cells.
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| Overall design |
AGM regions were obtained from E11.5 embryos by dissection and digested with 0.1% collagenase. Cells were stained with anti-CD31, anti-CD45 and anti-Ter119 antibodies. Sorting of the CD31+CD45-Ter119- population was performed and some cells were separated into ckit+ and ckit- (data previously submited GSE35395). The rest of the CD31+CD45- cells were incubated for 2 hours on OP9 stromal cells expressing ectopic Jagged1 or Delta4 (OP9-Jag1 and OP9-Dll4). After culture with each ligand, cells were separated into CD31+CD45-ckit+ and CD31+CD45-ckit-. Cells were harvested for RNA puriication and microarray analysis.
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| Contributor(s) |
Bigas A, Islam AB, Guiu J, Gama-Norton L, Espinosa L |
| Citation(s) |
26465397 |
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| Submission date |
Jul 11, 2014 |
| Last update date |
Mar 30, 2020 |
| Contact name |
Anna Bigas |
| E-mail(s) |
abigas@imim.es
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| Phone |
+34933160440
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| Organization name |
Institut Hospital del Mar d'Investigacions Mèdiques
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| Department |
Cancer Research
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| Lab |
Stem Cells and Cancer
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| Street address |
Dr. Aiguader 88
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| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (17)
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| GSM1435429 |
Ckit negative (Ckit-) cells at time T0, biological rep1 |
| GSM1435430 |
Ckit negative (Ckit-) cells at time T0, biological rep2 |
| GSM1435431 |
Ckit negative (Ckit-) cells at time T0, biological rep3 |
| GSM1435432 |
Ckit positive (Ckit+) cells at time T0, biological rep1 |
| GSM1435433 |
Ckit positive (Ckit+) cells at time T0, biological rep2 |
| GSM1435434 |
Ckit positive (Ckit+) cells at time T0, biological rep3 |
| GSM1435435 |
Ckit negative (Ckit-) cells at time T2 on OP9-Jag1, biological rep1 |
| GSM1435436 |
Ckit negative (Ckit-) cells at time T2 on OP9-Jag1, biological rep2 |
| GSM1435437 |
Ckit negative (Ckit-) cells at time T2 on OP9-Dll4, biological rep1 |
| GSM1435438 |
Ckit negative (Ckit-) cells at time T2 on OP9-Dll4, biological rep2 |
| GSM1435439 |
Ckit negative (Ckit-) cells at time T2 on OP9-Dll4, biological rep3 |
| GSM1435440 |
Ckit positive (Ckit+) cells at time T2 on OP9-Jag1, biological rep1 |
| GSM1435441 |
Ckit positive (Ckit+) cells at time T2 on OP9-Jag1, biological rep2 |
| GSM1435442 |
Ckit positive (Ckit+) cells at time T2 on OP9-Jag1, biological rep3 |
| GSM1435443 |
Ckit positive (Ckit+) cells at time T2 on OP9-Dll4, biological rep1 |
| GSM1435444 |
Ckit positive (Ckit+) cells at time T2 on OP9-Dll4, biological rep2 |
| GSM1435445 |
Ckit positive (Ckit+) cells at time T2 on OP9-Dll4, biological rep3 |
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| Relations |
| BioProject |
PRJNA255109 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59344_RAW.tar |
64.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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