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Series GSE59114 Query DataSets for GSE59114
Status Public on Sep 30, 2015
Title Single cell RNA_Seq in aging of hematopoietic stem and progenitor cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here, we use single-cell RNA-Seq to examine variation between individual hematopoietic stem and progenitor cells from two mouse strains (C57BL/6 and DBA/2) as they age. We prepared libraries from long-term (LT-HSCs) (LSK CD150+CD48-), short-term hematopoietic stem cells (ST-HSCs) (LSK CD150-CD48-) and multipotent progenitors (MPPs) (LSK CD150+CD48+) from young (2-3 months) and old mice (22 months for C57BL/6 and 20 months for DBA/2). Population controls for each cell type and age were isolated by sorting processed in parallel.
Overall design RNA seq libraries from single cells and population controls. All prepared with SMARTer protocol for cDNA and amplification followed by Nextera protocol for library preparation.
Contributor(s) Kowalczyk MS
Citation(s) 26430063
Submission date Jul 07, 2014
Last update date May 15, 2019
Contact name Monika Sylwia Kowalczyk
Organization name Broad Institute
Lab Regev Lab
Street address 415 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2128)
GSM1433164 young_C57BL6_LTHSC_1
GSM1433165 young_C57BL6_LTHSC_2
GSM1433166 young_C57BL6_LTHSC_3
BioProject PRJNA254994
SRA SRP044256

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59114_C57BL6_GEO_all.xlsx 98.1 Mb (ftp)(http) XLSX
GSE59114_DBA_GEO_all.xlsx 44.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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