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| Status |
Public on Dec 07, 2015 |
| Title |
Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [Mnase-seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Meiotic recombination hotspots are associated with histone post-translational modifications and open chromatin. However, it remains unclear how histone modifications and chromatin structure directly regulate meiotic recombination. Here, we identify acetylation of histone H4 at Lys44 (H4K44ac) as a new histone modification, occurring on the nucleosomal lateral surface. We show that H4K44ac is specific to yeast sporulation, rising during yeast meiosis and displaying genome-wide enrichment at recombination hotspots in meiosis. The H4K44 residue is required for normal meiotic recombination, for normal levels of double strand breaks during meiosis, and for optimal sporulation. Non-modifiable substitution H4K44R results in reduced MNase digestion and decreased binding of recombination-associated proteins at hotspots. Our results show that H4K44ac creates an accessible chromatin environment for key proteins to facilitate meiotic recombination.
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| Overall design |
Two samples, one WT MNase-seq and one MNase-seq from yeast with a lysine->arginine mutation at H4K44, no replicates
Two replicates each of MNase-seq in WT and H4K44->R mutant yeast grown in YPD or YPA.
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| Contributor(s) |
Hu J, Donahue G, Dorsey J, Govin J, Shah PP, Yuan Z, Garcia BA, Berger SL |
| Citation(s) |
26628362 |
| |
| Submission date |
Jul 01, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Gregory Donahue |
| Organization name |
The University of Pennsylvania
|
| Department |
Cell & Developmental Biology
|
| Lab |
Zaret Lab
|
| Street address |
3400 Civic Center Blvd, Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
|
| Samples (10)
|
| GSM1849298 |
MNase-seq in WT yeast grown in YPD, rep #2 |
| GSM1849299 |
MNase-seq in H4K44->R mutant yeast grown in YPD, rep #1 |
| GSM1849300 |
MNase-seq in H4K44->R mutant yeast grown in YPD, rep #2 |
| GSM1849301 |
MNase-seq in WT yeast grown in YPA, rep #1 |
| GSM1849302 |
MNase-seq in WT yeast grown in YPA, rep #2 |
| GSM1849303 |
MNase-seq in H4K44->R mutant yeast grown in YPA, rep #1 |
| GSM1849304 |
MNase-seq in H4K44->R mutant yeast grown in YPA, rep #2 |
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| This SubSeries is part of SuperSeries: |
| GSE59005 |
Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin |
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| Relations |
| BioProject |
PRJNA254081 |
| SRA |
SRP043975 |
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