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Series GSE58993 Query DataSets for GSE58993
Status Public on Aug 08, 2014
Title RNA-seq of 8hr LPS-stimulated bone marrow-derived macrophages from wild type, Ch25h-deficient, and LXRab knockout mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary 25-hydroxycholesterol has been demonstrated to regulate SREBP processing, yet Ch25h-deficient mice have no cholesterol abnormalities. Using RNA-seq, we find that LPS-stimulated, Ch25h-deficient BMDMs have dysregulated SREBP target genes, demonstrating that 25-hydroxycholesterol is an induced repressor of SREBP during inflammatory settings.
Overall design mRNA profiles from day 7 M-CSF bone marrow-derived macrophages (wild type, Ch25h-knockout, LXR-double knockout, were generated by deep sequencing on an Illumina HiSeq 2500. Sequence reads passing a quality filter were aligned to the mouse genome (mm10) using STAR.
Contributor(s) Dang E, Reboldi A, Cyster J
Citation(s) 25104388
Submission date Jul 01, 2014
Last update date May 15, 2019
Contact name Jason Cyster
Organization name University of California - San Francisco
Street address 513 Parnassus Avenue
City San Francisco
State/province California
ZIP/Postal code 94143
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1423551 WT_1
GSM1423552 WT_2
GSM1423553 Ch25hKO_1
BioProject PRJNA254064
SRA SRP043965

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Supplementary file Size Download File type/resource
GSE58993_CysterlabRNAseq.txt.gz 257.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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