|
| Status |
Public on Dec 01, 2014 |
| Title |
Chromatin-dependent regulation of the RNA polymerases II and III activity throughout the transcription cycle |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by genome tiling array
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
We have developed a new genome-wide protocol for nascent transcription analysis at high resolution in the yeast Saccharomyces cerevisiae. This protocol is based in run-on labeling of nascent RNA with a biotinylated precursor. We call it BioGRO for biotin-based genomic run-on.
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| |
|
| Overall design |
7 samples total; 5 BioGRO samples (4 mutants and 1 WT); and 2 ChIP-chip samples (Rpb3 IP and its input).
|
| Web link |
http://www.uv.es/gfl/
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| |
|
| Contributor(s) |
Jordán-Pla A, Perez-Ortán JE, de-Miguel L, Chávez S, Pelechano V |
| Citation(s) |
25550430, 26483020, 29212533 |
| |
| Submission date |
Jun 26, 2014 |
| Last update date |
Aug 31, 2019 |
| Contact name |
Jose E. Perez-Ortin |
| E-mail(s) |
jose.e.perez@uv.es
|
| Phone |
34 963 543467
|
| Organization name |
Universitat de Valencia
|
| Department |
Bioquimica y Biologia Molecular
|
| Lab |
Yeast Functional Genomics (GFL)
|
| Street address |
Dr. Moliner 50
|
| City |
Burjassot |
| State/province |
Valencia |
| ZIP/Postal code |
E46100 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL18871 |
Affymetrix/Steinmetz Saccharomyces cerevisiae custom tiling array |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA253732 |
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