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| Status |
Public on Sep 20, 2014 |
| Title |
PAX2-null progenitors define multiple lineages with common expression signatures in benign and neoplastic oviductal epithelium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The oviducts contain high grade serous cancer precursors, which are γ-H2AXp and p53 mutation positive. Secretory cell outgrowths (SCOUTs) are associated with older age and serous cancer. We evaluated PAX2 expression in proliferating oviductal cells, normal mucosa, SCOUTs, Walthard cell nests, STINs and HGSCs. Non-ciliated cells in normal mucosa were PAX2 positive but became PAX2 negative in multilayered epithelium. PAX2 negative SCOUTs fell into two groups; Type I were secretory or secretory/ciliated with a “tubal” phenotype and were ALDH1 negative. Type II displayed a columnar to pseudostratified phenotype, with an EZH2,ALDH1, β-catenin, Stathmin, LEF1, RCN1 and RUNX2 expression signature . This study, for the first time, links PAX2 negative with proliferating fetal and adult oviductal cells undergoing basal and ciliated differentiation and shows that this expression state is maintained in SCOUTs, STINs and HGSCs. All three entities can demonstrate a consistent perturbation of genes involved in potential tumor suppressor gene silencing (EZH2), transcriptional regulation (LEF1), regulation of differentiation (RUNX2) calcium binding (RCN1) and oncogenesis (Stathmin). This shared expression signature between benign and neoplastic entities links normal progenitor cell expansion to abnormal and neoplastic outgrowth in the oviduct and exposes a common pathway that could be a target of early prevention.
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| Overall design |
17 total samples are analyzed. 4 for TYPE 1 SCOUTs, 3 for TYPE 2 SCOUTs, 7 for paired normal epithelium, 3 for tumor
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| Contributor(s) |
Ning G, Crum CP, McKeon FD, Xian W |
| Citation(s) |
25130537 |
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| Submission date |
Jun 26, 2014 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Frank McKeon |
| E-mail(s) |
mckeon.xian@gmail.com
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| Phone |
8608372486
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| Organization name |
University of Houston
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| Department |
Biology and Biochemistry
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| Street address |
3455 Cullen Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA253680 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58842_RAW.tar |
326.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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