GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE58651 Query DataSets for GSE58651
Status Public on Jun 20, 2014
Title Epigenome Wide Association Study unveils differential methylation of Leukocyte cell adhesion molecules in neonatal sepsis
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary DNA methylation is the current strategy in the field of biomarker discovery due to its prognostic efficiency. Its role in prognosis and early diagnosis has been recognized in various types of cancer. Sepsis still remains one of the major causes of neonatal mortality due to the lack of sensitive diagnostic and prognostic biomarkers. Delay in sepsis diagnosis leads to treatment difficulties and poor outcomes. In this study, we have done an epigenome wide search to identify potential markers for prognosis of neonatal sepsis which may improve the treatment strategies. Illumina 450K methylation microarray revealed that the genes involved in transendothelial leukocyte migration were differentially methylated in septic newborns compared to non-septic newborns, especially the Protocadherin Beta group. Genes like ITGB2-AS1, CCS were found to be differentially methylated significantly, which gives the hope of developing novel, potential epigenetic markers for neonatal sepsis. From this study, we conclude that DNA methylation might play crucial functions in the pathophysiology of neonatal sepsis which was obvious from the difference in methylation level among septic and non-septic babies. In future, the potentiality of these epigenetic biomarkers can be studied in large scale with appropriate techniques which will give further in depth knowledge in this context.
Overall design DNA methylation analysis of three septic newborns and three non-septic newborns were performed with Illumina Infinium HumanMethylation450 BeadChip. Peripheral venous blood sample was collected from the babies during the third day of birth while taking blood for routine investigations. Non-septic babies are babies admitted to NICU and sampled for other minor ailments. Genomic DNA was extracted using QIAmp DNA Blood Mini kit (Qiagen, Hilden, Germany) and bisulfite treated using EZ DNA methylation kit (Zymoresearch, USA).
Contributor(s) Dhas BB, Ashmi H, Bhat V, Parija SC
Citation(s) 26484145
Submission date Jun 19, 2014
Last update date Mar 22, 2019
Contact name Benet Bosco Dhas
Phone +918508406015
Organization name Jawaharlal Insitute of Postgraduate Medical Education and Research
Department Paediatrics
Lab Genomics
Street address Danvantri Nagar
City Pondicherry
State/province Pondicherry
ZIP/Postal code 605006
Country India
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (6)
GSM1416379 C30
GSM1416380 C33
GSM1416381 C50
BioProject PRJNA253101

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58651_RAW.tar 183.1 Mb (http)(custom) TAR
GSE58651_Raw_data.txt.gz 28.3 Mb (ftp)(http) TXT
GSE58651_Results.txt.gz 124.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap