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| Status |
Public on Dec 31, 2015 |
| Title |
Gene expression signature associated with BRAFV600E mutation in human papillary thyroid carcinoma based on transgenic mouse model (human) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
BRAFV600E mutation is the most frequent molecular event in papillary thyroid carcinoma. The relation of this genetic alteration with the factors od poor prognosis has been reported as well as its influence on PTC gene signature. However human material disables distinction of cancer causes from its effect.
We used our transgenic mouse model of papillary thyroid carcinoma induced by the BRAFV600E mutation to select BRAFV600E-specifi gene signature which was than compared to human material
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| Overall design |
The human microarray data were obtained for: 27 papillary thyroid carcinomas including 18 BRAF(+), 8 RET(+), 1 RAS(+); 18 apparently healthy thyroids. In order to find BRAFV600E-specific gene signature data obtained from our transgenic mouse model of PTC were compared to human material. The analyses were performed taking into account not only the BRAF mutation but also other PTC initiating events including RET rearrangements and RAS poin mutations. Morover the microarray data were validated with the QPCR reaction.
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| Contributor(s) |
Rusinek D, Swierniak M, Jarzab B |
| Citation(s) |
26625260 |
| |
| Submission date |
Jun 16, 2014 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Michal Swierniak |
| Organization name |
University of Warsaw
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| Department |
Centre of New Technologies
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| Street address |
Banacha 2C
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| City |
Warsaw |
| ZIP/Postal code |
02-097 |
| Country |
Poland |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (45)
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| This SubSeries is part of SuperSeries: |
| GSE58689 |
Gene expression signature associated with BRAFV600E mutation in human papillary thyroid carcinoma based on transgenic mouse model |
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| Relations |
| BioProject |
PRJNA252852 |
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