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| Status |
Public on Jun 20, 2014 |
| Title |
Expression data of wild type and C-DGCR8 KO cones at different timepoints [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have analyzed gene expression in cone photoreceptors isolated from wild type and C-DGCR8 (DiGeorge Syndrome Critical Region Gene 8) KO mice at five different time points to get a mechanistic inside into the altered molecular pathways after microRNAs depletion.
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| Overall design |
Cones were isolated by FACS from wild type and C-DGCR8 KO mice expressing Cre recombinase postnatally specifically in cones (D4-Cre) and bearing conditional null Dgcr8 allele. Experiments were performed in duplicates from different time points (P30, P40, P50, P60, and P90). Total RNA was extracted and next-generation RNA sequencing was performed.
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| Contributor(s) |
Krol J, Busskamp V, Roska B, Filipowicz W |
| Citation(s) |
25002228 |
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| Submission date |
Jun 13, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Tim Roloff |
| E-mail(s) |
tim.roloff@fmi.ch
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| Organization name |
FMI
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| Department |
Functional Genomics
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| Street address |
Maulbeerstrasse 66
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| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (20)
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| GSM1416775 |
P40_wt cones, replicate 2 [RNA-seq] |
| GSM1416776 |
P50_wt cones, replicate 1 [RNA-seq] |
| GSM1416777 |
P50_wt cones, replicate 2 [RNA-seq] |
| GSM1416778 |
P60_wt cones, replicate 1 [RNA-seq] |
| GSM1416779 |
P60_wt cones, replicate 2 [RNA-seq] |
| GSM1416780 |
P90_wt cones, replicate 1 [RNA-seq] |
| GSM1416781 |
P90_wt cones, replicate 2 [RNA-seq] |
| GSM1416782 |
P30_C-DGCR8 KO cones, replicate 1 [RNA-seq] |
| GSM1416783 |
P30_C-DGCR8 KO cones, replicate 2 [RNA-seq] |
| GSM1416784 |
P40_C-DGCR8 KO cones, replicate 1 [RNA-seq] |
| GSM1416785 |
P40_C-DGCR8 KO cones, replicate 2 [RNA-seq] |
| GSM1416786 |
P50_C-DGCR8 KO cones, replicate 1 [RNA-seq] |
| GSM1416787 |
P50_C-DGCR8 KO cones, replicate 2 [RNA-seq] |
| GSM1416788 |
P60_C-DGCR8 KO cones, replicate 1 [RNA-seq] |
| GSM1416789 |
P60_C-DGCR8 KO cones, replicate 2 [RNA-seq] |
| GSM1416790 |
P90_C-DGCR8 KO cones, replicate 1 [RNA-seq] |
| GSM1416791 |
P90_C-DGCR8 KO cones, replicate 2 [RNA-seq] |
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| This SubSeries is part of SuperSeries: |
| GSE58501 |
MiRNAs 182 and 183 are necessary to maintain adult cone photoreceptor outer segments and visual function |
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| Relations |
| BioProject |
PRJNA253146 |
| SRA |
SRP043436 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58498_RAW.tar |
2.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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