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Series GSE58283 Query DataSets for GSE58283
Status Public on Nov 01, 2014
Title Gene expression profiling of mouse bone marrow-derived macrophages (RNA-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Bone marrow (BM) cells were obtained by flushing the long bones of 8-week old C57BL/6 mice. BM cells were then were plated in macrophage SFM medium (Life Technologies) supplemented with penicillin-streptomycin and CSF-1 (Peprotech, 100 ng/ml) and cultured for one week to allow macrophage differentiation. BMDMs were polarized by adding IL-4 to the medium (40 ng/ml, Peprotech) for 72h or left untreated.
Overall design 8 samples total, 2 groups, 4 biological replicates in each group
Contributor(s) Squadrito ML, Baer C, Burdet F, Maderna C, Gilfillan GD, Lyle R, Ibberson M, De Palma M
Citation(s) 25159140
Submission date Jun 06, 2014
Last update date May 15, 2019
Contact name Mark Ibberson
Organization name SIB Swiss Institute of Bioinformatics
Department Vital-IT
Street address Genopode building
City Lausanne
ZIP/Postal code CH-1015
Country Switzerland
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM1405837 BMDM_1
GSM1405838 BMDM_2
GSM1405839 BMDM_3
BioProject PRJNA251892
SRA SRP043011

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58283_gene_counts.txt.gz 324.6 Kb (ftp)(http) TXT
GSE58283_merged.gtf.gz 9.5 Mb (ftp)(http) GTF
GSE58283_merged_isoforms_FPKM.txt.gz 2.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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