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| Status |
Public on Jan 01, 2016 |
| Title |
Expression data from primary term human decidual cells treated with interleukin-1-beta for 6 hours. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Preterm birth is an important unsolved clinical problem. Despite advanced treatments, infants who survive prematurity remain at increased risk for permanent disabilities. In approximately one-third of cases, prematurity is related to infection and/or inflammation, which renders hostile the normally receptive intrauterine environment. Proinflammatory cytokines provoke up-regulation of genes that promote uterine contractions. Using monolayer cultures of human decidual cells as a model, we profiled the global pattern of gene expression in response to cytokine challenge.
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| Overall design |
Decidualized human endometrial stromal cells were treated with 1 ng/ml of interleukin-1-beta for 6 hours, while control cells were treated with 0.1% PBS containing 0.1% bovine serum albumin (vehicle). The cells were subjected to RNA extraction, quantification, and evaluation, followed by hybridization on Affymetrix Human Gene 2.0 ST Arrays (Platform GPL16686). Three biological replicates were used (6 total samples).
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| Contributor(s) |
Ibrahim SA, Ackerman WE, Summerfield TL, Lockwood CJ, Kniss DA |
| Citation(s) |
26348374 |
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| Submission date |
Jun 04, 2014 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Douglas A Kniss |
| E-mail(s) |
kniss.1@osu.edu
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| Phone |
614-293-4496
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| Organization name |
Ohio State University
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| Department |
Obstetrics and Gynecology
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| Lab |
Perinatal Research Lab
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| Street address |
395 W. 12th Ave
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| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM1404200 |
decidual cells, vehicle, biological rep1 |
| GSM1404201 |
decidual cells, interleukin-1-beta, biological rep1 |
| GSM1404202 |
decidual cells, vehicle, biological rep2 |
| GSM1404203 |
decidual cells, interleukin-1-beta, biological rep2 |
| GSM1404204 |
decidual cells, vehicle, biological rep3 |
| GSM1404205 |
decidual cells, interleukin-1-beta, biological rep3 |
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| Relations |
| BioProject |
PRJNA251636 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58220_RAW.tar |
52.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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