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Series GSE57745 Query DataSets for GSE57745
Status Public on Jun 01, 2015
Title Transposon expression kinetics in Dnmt3L-/- developing testes [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We examined the kinetics of production of mRNAs and small RNAs derived from transposable elements during mouse spermatogenesis, in whole gonads of wildtype and DNA methylation-deficient males (Dnmt3L and Miwi2 mutants). We found that in absence of DNA methylation, transposon reactivation is not constitutive but rather occurs in a class- and development-specific manner : both the intensity of reactivation and the number of reactivated transposon classes increased as germ cells progress in meiosis. Moreover, we observed that transposon silencing before meiosis is not due to increased cleavage by the piRNA machinery. In contrast, the burst of transposon transcripts occurring at meiosis in the absence of DNA methylation serve as substrates for increased piRNA production
Overall design Six whole testis samples were analyzed, corresponding to three time points (16.5dpc, 10dpp and 20dpp) each for Dnamt3L-/- animals and control littermates. For 16.5dpc, testes from 7/8 mice were pooled per genotype. For the other stages, three mice were pooled per genotype.
Contributor(s) Bourc'his D
Citation(s) 26109049
Submission date May 16, 2014
Last update date May 15, 2019
Contact name Nicolas Servant
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1387944 RNA Seq Dnmt3L HET 16.5dpc
GSM1387945 RNA Seq Dnmt3L KO 16.5dpc
GSM1387946 RNA Seq Dnmt3L HET 10dpp
This SubSeries is part of SuperSeries:
GSE57747 Kinetics of transposon-derived transcripts and piRNAs in the absence of DNA methylation during mouse spermatogenesis
BioProject PRJNA247988
SRA SRP042045

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57745_RAW.tar 70.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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