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| Status |
Public on Apr 01, 2015 |
| Title |
Expression analysis of p53-/- and p53-/-, Ha-RasV12-transformed MEFs upon E4F1 gene inactivation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
E4F1 is a ubiquitously expressed zinc-finger protein of the Gli-Kruppel family that was first identified, more than 30 years ago, as a cellular target of the adenoviral oncoprotein E1A13S (Ad type V), required for transcriptional regulation of adenoviral genes. In order to identify the p53-independent program controlled by E4F1, we performed microarray analyses in p53 KO and p53 KO; Ha-RasV12-transformed mouse embryonic fibroblasts (MEFs) in wild type and E4F1-inactivated cells.
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| Overall design |
To address p53-independent E4F1 transcriptome, a 12 chip array study has been realized using total RNA recovered from wild-type (E4F1+/flox, CRE infected; odd Samples) MEFs and E4F1-depleted (E4F1-/flox, CRE infected; even Samples) MEFs in p53-/- (Samples 1 to 12) and p53-/-, Ha-RasV12 background (Samples 13 to 24). p53-/- MEFs were derived from 13.5-day mouse embryos. Transformed p53-/- MEFs were generated by infection with a recombinant retrovirus encoding for Ha-RasV12. Three independent biological replicates of wild-type and knock-out MEFs for E4F1 have been used on the two genetic backgrounds.
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| Contributor(s) |
Rodier G, Kirsh O, Sardet C |
| Citation(s) |
25843721, 27621446, 27621431 |
| |
| Submission date |
May 01, 2014 |
| Last update date |
Sep 22, 2016 |
| Contact name |
Claude Sardet |
| E-mail(s) |
claude.sardet@inserm.fr
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| Organization name |
Institut de Recherche en Cancérologie de Montpellier (IRCM)
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| Department |
Inserm U1194 - Université de Montpellier - Institut Régional du Cancer de Montpellier (ICM)
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| Street address |
208 rue des Apothicaires
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| City |
Montpellier |
| ZIP/Postal code |
34298 |
| Country |
France |
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| Platforms (1) |
| GPL15902 |
NimbleGen Mouse gene expression array [100718_MM9_EXP] (probe-level version) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE57242 |
The transcription factor E4F1 coordinates CHK1-dependent checkpoint and mitochondrial functions |
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| Relations |
| BioProject |
PRJNA246033 |
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