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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 09, 2015 |
| Title |
Cpeb4-mediated Translational Regulatory Circuitry Controls Terminal Erythroid Differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Erythropoiesis is essential to mammals and is regulated at multiple steps by both extracellular and intracellular factors. Many transcriptional regulatory networks in erythroid differentiation have been well characterized. However, our understanding of post-transcriptional regulatory circuitries in this developmental process is still limited. Using genomic approaches, we identified a sequence-specific RNA-binding protein, Cpeb4, which is dramatically induced in terminal erythroid differentiation (TED) by two erythroid important transcription factors, Gata1/Tal1. Cpeb4 belongs to the cytoplasmic polyadenylation element binding (CPEB) protein family that regulates translation of target mRNAs in early embryonic development, neuronal synapse, and cancer. Using primary mouse fetal liver erythroblasts, we found that Cpeb4 is required for terminal erythropoiesis by repressing the translation of a set of mRNAs highly expressed in progenitor cells. This translational repression occurs by the interaction with a general translational initiation factor, eIF3. Interestingly, Cpeb4 also binds its own mRNA and represses its translation, thus forming a negative regulatory circuitry to limit Cpeb4 protein level. This mechanism ensures that the translation repressor, Cpeb4, does not interfere with the translation of other mRNAs in differentiating erythroblasts. Our study characterized a translational regulatorycircuitry that controls TED and revealed that Cpeb4 is required for somatic cell differentiation.
We used microarray to identify mRNAs associated with Cpeb4 in mouse fetal liver erythroblasts.
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| Overall design |
Cpeb4 associated mRNAs were isolated from mouse fetal liver erythroblasts using anti-Cpeb4 antibody for immunoprecipitation followed by RNA extraction. Then Affymetrix microarrays were used to identify and quantify the mRNAs associated with Cpeb4.
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| Contributor(s) |
Hu W, Yuan B |
| Citation(s) |
25220394 |
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| Submission date |
Apr 23, 2014 |
| Last update date |
Dec 09, 2015 |
| Contact name |
Wenqian Hu |
| Organization name |
Mayo Clinic
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| Department |
Biochemistry and Molecular Biology
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| Street address |
200 First St SW, GU16-01A
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| City |
Rochester |
| State/province |
Minnesota |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL17791 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [mogene20st_Mm_ENTREZG_17.1.0] |
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| Samples (4)
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| GSM1372824 |
RNA isolated from total Input |
| GSM1372825 |
RNA isolated from IgG control |
| GSM1372826 |
RNA isolated from Cpeb4 immunoprecipitation, biological replica 1 |
| GSM1372827 |
RNA isolated from Cpeb4 immunoprecipitation, biological replica 2 |
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| Relations |
| BioProject |
PRJNA245198 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57004_RAW.tar |
52.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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