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Series GSE56525 Query DataSets for GSE56525
Status Public on Jan 15, 2015
Title Distinct human stem cell populations in small and large intestine
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The intestine is composed of an epithelial layer, containing rapidly proliferating cells that mature into two distinct anatomic regions, the small and the large intestine. Although previous studies have identified stem cells as the cell-of-origin for the whole intestine, no studies have compared stem cells derived from the small and large intestine. Here, we report intrinsic differences between these two populations of cells.  Primary epithelial cells isolated from human fetal small and large intestine and expanded with Wnt agonist, R-spondin 2, displayed differential expression of stem cell markers and separate hierarchical clustering of gene expression involved in differentiation, proliferation and disease pathways. Using a three-dimensional in vitro differentiation assay, single cells derived from small and large intestine formed distinct organoid architecture with cellular hierarchy similar to that found in primary tissue. Our characterization of human fetal intestinal stem cells defies the classical definition proposed by most where small and large intestine are repopulated by an identical epithelial stem cell and raises the question of the importance of intrinsic and extrinsic cues in the development of intestinal diseases. 
Overall design 12 samples were analyzed. They consisted of human fetal small and large intestine (SI; n=6 and LI; n=6) stem cells, expanded with Wnt agonist and R-spondin 2. Differential expression of genes in epithelial cells from both the large and small intestine were observed.
Contributor(s) LaFramboise W, Cramer J
Citation(s) 25751518
Submission date Apr 04, 2014
Last update date Dec 06, 2018
Contact name William Armand LaFramboise
Phone 412-623-6160
Organization name University of Pittsburgh
Department Pathology
Street address 5230 Centre Avenue
City Pittsburgh
State/province PA
ZIP/Postal code 15232
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM1363317 LI2
GSM1363318 LI24
GSM1363319 LI27
BioProject PRJNA243625

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Supplementary file Size Download File type/resource
GSE56525_RAW.tar 22.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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