|
| Status |
Public on Mar 20, 2014 |
| Title |
in vitro differentiated Th0, Th17, and Tr1 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression profiling of in vitro differentiated murine Th cell subsets. Flow cytometrically sorted naive Th cells (CD4+ CD44- Foxp3-) were polyclonally stimulated in vitro for 3 days using 4 µg/ml plate-bound antibody to CD3 (145-2C11) and 2 µg/ml soluble antibody to CD28 (PV-1).
Th0 cells were cultured in the absence of exogenous cytokines. Th17 cells were differentiated with 50 ng/ml IL-6 plus 0.5 ng/ml TGF-β. Tr-1 cells were differentiated with 100 ng/ml IL-27 plus 0.5 ng/ml TGF-β.
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| |
|
| Overall design |
Five biological replicates per Th subset (Th0, Th17, Tr1)
|
| |
|
| Contributor(s) |
Korn T, Heink S, Heinemann C |
| Citation(s) |
24796719, 35819408 |
| |
| Submission date |
Mar 19, 2014 |
| Last update date |
Aug 24, 2022 |
| Contact name |
Thomas Korn |
| Organization name |
TU Munich, Klinikum rechts der Isar
|
| Department |
Neurology
|
| Lab |
Neuroimmunology
|
| Street address |
Ismaninger Str. 22
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA242233 |
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