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| Status |
Public on Dec 31, 2016 |
| Title |
Elucidating drug mechanistics and resistance in HER2-positive breast cancer cell lines by mathematical modeling |
| Organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
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| Summary |
Advancing precision medicine in the field of cancer is still curbed by the fact that a rational basis to predict treatment outcome is missing. To unravel the mechanism behind targeted drugs (trastuzumab, pertuzumab, erlotinib), mathematical modeling employing ordinary differential equations (ODE) was combined with wet-lab experimentation. Experimentation relied on systematic perturbation experiments to monitor the signaling-response towards drugs in the context of EGF signaling in the HER2+ cell lines SKBR3 and HCC1954.
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| Overall design |
The impact of three different targeted drugs (trastuzumab, pertuzumab, erlotinib) on the breast cancer cell lines SKBR3 and HCC1954 was analyzed in a time-resolved manner. Each perturbation was performed as biological triplicate and the expression level of 31 different protein/phosphoproteins was monitored using reverse phase protein arrays. The selected protein/phosphoproteins are all part of the EGF signaling pathway.
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| Contributor(s) |
Kaschek D, Sonntag J, Henjes F, von der Heyde S, Quack C, Wiemann S, Hasmann M, Beißbarth T, Korf U, Timmer J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 13, 2014 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Ulrike Korf |
| E-mail(s) |
u.korf@dkfz.de
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| Phone |
0049 (0) 6221 424765
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| Fax |
0049 (0) 6221 423454
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| Organization name |
German Cancer Research Center
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| Department |
Molecular Genome Analysis
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| Street address |
Im Neuenheimer Feld 580
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA241254 |
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