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Series GSE55823 Query DataSets for GSE55823
Status Public on Mar 13, 2014
Title ERK Oscillation-Dependent Gene Expression Patterns and Deregulation By The Stress-Response
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Studies were undertaken to determine whether oscillatory behavior in the extracellular signal regulated kinase (ERK) pathway results in unique gene regulation patterns. Microarray analysis was performed on three subcloned populations of human keratinocytes with distinct ERK signaling/oscillation phenotypes.
Microarray analysis identified 45 genes that overlapped between 2 subclones with oscillation phenotypes but not in the subclone which is non-oscillatory. Transcription factor networks revealed a role for MED1 in mediating ERK oscillation-dependent gene expression, which was confirmed with Western blot analysis. Further experimentation confirmed a role for p38 in the mediation of MED1 phosphorylation and ERK oscillatory behavior.
Overall design hTERT-immortalized normal human keratinocytes (provided by Dr. Jerry Shay, The University of Texas Southwestern Medical Center) were stably transfected with ERK1-green fluorescent protein chimera and stable subclones were isolated with distinct ERK activation/oscillation patterns: Clone #1 exhibits transient ERK activation with ligand activation but does not oscillate; Clone #2 exhibits persistent ERK oscillations that are dependent on ligand activation; and Clone #3 exhibits spontaneous ERK oscillations in the absence of ligand activation.
Contributor(s) Waters KM, Weber TJ
Citation(s) 25068892
Submission date Mar 12, 2014
Last update date Dec 06, 2018
Contact name Katrina M Waters
Organization name Pacific Northwest National Laboratory
Department Biological Sciences Division
Street address 902 Battelle Blvd; MSIN J4-18
City Richland
State/province WA
ZIP/Postal code 99352
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (30)
GSM1346499 Clone#2_TGFα_rep1
GSM1346500 Clone#2_TGFα_rep2
GSM1346501 Clone#2_TGFα_rep3
BioProject PRJNA241079

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Supplementary file Size Download File type/resource
GSE55823_RAW.tar 61.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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