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| Status |
Public on Mar 13, 2014 |
| Title |
ERK Oscillation-Dependent Gene Expression Patterns and Deregulation By The Stress-Response |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Studies were undertaken to determine whether oscillatory behavior in the extracellular signal regulated kinase (ERK) pathway results in unique gene regulation patterns. Microarray analysis was performed on three subcloned populations of human keratinocytes with distinct ERK signaling/oscillation phenotypes.
Microarray analysis identified 45 genes that overlapped between 2 subclones with oscillation phenotypes but not in the subclone which is non-oscillatory. Transcription factor networks revealed a role for MED1 in mediating ERK oscillation-dependent gene expression, which was confirmed with Western blot analysis. Further experimentation confirmed a role for p38 in the mediation of MED1 phosphorylation and ERK oscillatory behavior.
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| Overall design |
hTERT-immortalized normal human keratinocytes (provided by Dr. Jerry Shay, The University of Texas Southwestern Medical Center) were stably transfected with ERK1-green fluorescent protein chimera and stable subclones were isolated with distinct ERK activation/oscillation patterns: Clone #1 exhibits transient ERK activation with ligand activation but does not oscillate; Clone #2 exhibits persistent ERK oscillations that are dependent on ligand activation; and Clone #3 exhibits spontaneous ERK oscillations in the absence of ligand activation.
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| Contributor(s) |
Waters KM, Weber TJ |
| Citation(s) |
25068892 |
| |
| Submission date |
Mar 12, 2014 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Katrina M Waters |
| E-mail(s) |
katrina.waters@pnl.gov
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| Organization name |
Pacific Northwest National Laboratory
|
| Department |
Biological Sciences Division
|
| Street address |
902 Battelle Blvd; MSIN J4-18
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| City |
Richland |
| State/province |
WA |
| ZIP/Postal code |
99352 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA241079 |
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