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| Status |
Public on Mar 12, 2014 |
| Title |
Expression data from 26972c yeast ± bHLHm1 (SAT1) |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
26972c yeast cells were transformed with either empty vector (pYES3) or pYES3:Gm:bHLHm1. Cells were grown on low ammonium concentrations to observe transcriptional changes in the yeast genome in response to the soybean bHLHm1 transcription factor.
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| Overall design |
The ammonium transport mutant (26972c) was transformed with either a empty vector control (pYES3) or pYES3 containing GmbHLHm1.Independent replicates (3) for each treatment were grown for 12 hours in the presence of 1 mM ammonium and 2 % w/vol galactose to activate the Gal promoter located on pYES3. Cells were harvested and total RNA extracted. Quantified RNA was analaysed using microarray analysis using the [Yeast_2] Affymetrix Yeast Genome 2.0 Array.
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| Contributor(s) |
Kaiser B |
| Citation(s) |
24707045 |
| Submission date |
Mar 11, 2014 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Brent N Kaiser |
| E-mail(s) |
brent.kaiser@adelaide.edu.au
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| Phone |
+61883136650
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| Organization name |
The University of Adelaide
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| Department |
School of Agriculture Food and Wine
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| Street address |
Waite Campus
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| City |
Urrbrae |
| State/province |
SA |
| ZIP/Postal code |
5064 |
| Country |
Australia |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA241026 |
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