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| Status |
Public on Apr 15, 2014 |
| Title |
Genome-wide comparative analysis of gene expression in wild type (WT3) and TRPM7-/- (K09) mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
TRPM7 is a ubiquitous ion channel and kinase, a unique ‘chanzyme’, required for proper early embryonic development. In order to assess the effects of TRPM7 activity on cellular gene expression, mouse embryonic stem cells with TRPM7 gene deletion (TRPM7-/- mESC) were established. By using microarray analysis, we identified genes with transcription significantly different in TRPM7-deficient mESC.
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| Overall design |
Total RNA was extracted from cells using Qiagen RNAeasy Plus mini Kit. Triplicate samples were made for each wild type clone 3 (WT3) and and TRPM7-/- clone 9 (KO9) mESC
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| Contributor(s) |
Clapham DE, Krapivinsky G |
| Citation(s) |
24855944 |
| |
| Submission date |
Mar 04, 2014 |
| Last update date |
Jan 16, 2019 |
| Contact name |
David E. Clapham |
| E-mail(s) |
dclapham@enders.tch.harvard.edu
|
| Organization name |
HHMI/Boston Children's Hospital/Harvard Medical School
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| Department |
Cardiology
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| Street address |
Enders 1309
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA240092 |
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