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Series GSE55434 Query DataSets for GSE55434
Status Public on Feb 28, 2014
Title Expression data from rat hepatocyte during liver regeneration after partial hepatectomy
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The recovery of liver mass is mainly mediated by proliferation and enlargement of hepatocytes after partial hepatectomy. Studying the gene expression profiles of hepatocytes after partial hepatectomy will be helpful in exploring the mechanism of liver regeneration.
We used microarrays to further highlight the regulatory role of hepatocyte in liver regeneration at gene transcription level.
 
Overall design Rat liver regeneration after partial hepatectomy (PH) is a good model to study the regulation of cell proliferation. We isolated hepatocytes from regenerating liver at 9 time points (2, 6, 12,24, 30, 36, 72, 120, and 168h) after PH and measured gene expression profiles of hepatocytes from 2h to 168h with rat Genome 230 2.0 gene chip. Each sample corresponding to one time point was hybridized onto one array. The experiment was repeated 3 times for each time point. In total, 10 time points were measured and 0h was used control group. After careful quality control analyses of each chip, Affymetrix GCOS 2.0 software was used to analyze the data. The relevance of gene expression profiles and biological processes was analyzed by bioinformatics and systems biology.
 
Contributor(s) Xu C, Zhang X, Wang G, Zhou Y
Citation(s) 29545936
Submission date Feb 27, 2014
Last update date Mar 20, 2018
Contact name Cunshuan Xu
E-mail(s) cellkeylab@126.com
Organization name Henan Normal University
Street address No. 46, Construction East Road
City Xinxiang
ZIP/Postal code 453007
Country China
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (57)
GSM1336621 Regenerating liver at 0h after PH_technical rep1
GSM1336622 Regenerating liver at 0h after PH_technical rep2
GSM1336623 Regenerating liver at 0h after PH_technical rep3
Relations
BioProject PRJNA239572

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55434_PHgroup_ratios.txt.gz 10.3 Mb (ftp)(http) TXT
GSE55434_RAW.tar 130.6 Mb (http)(custom) TAR (of CEL)
GSE55434_SOgroup_ratios.txt.gz 10.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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