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| Status |
Public on Feb 19, 2014 |
| Title |
Evaluation of chronic sublethal effects effects of the organophosphate flame retardant Tris(2-butoxyethyl)phosphate (TBEP) using Daphni magna transcriptomic response |
| Organism |
Daphnia magna |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of this study was to measure chronic sublethal effects of the flame retardant Tris(2-butoxyethyl)phosphate (TBEP) on gene expression, associated protein activity and life-history endpoints (reproduction, development) in the freshwater crustacean Daphnia magna. We exposed <24h old D. magna neonates for 21 days to culture medium (control) and to 3 sublethal doses of TBEP: 14.7, 147 and 1470 µg/L. Exposure doses were chosen from 48h acute toxicity testing and represent 1/10000, 1/1000 and 1/100 of the LC50, respectively. Each condition (control and the 3 different doses) were performed on 3 independant biological replicates, with 10 individuals each. We used a custom 15000 sequences D. magna microarray to measure transcriptional effect of each of the TBEP doses in comparison to unexposed controls.
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| Overall design |
We exposed <24h old Daphnia magna to culture medium (unexposed controls) and 3 sublethal doses of TBEP (14.7µg/L, 147µg/L and 1470µg/L) for 21 days. We used 3 biological replicates of 10 individuals for each condition.
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| Contributor(s) |
Giraudo M, Douville M, Houde M |
| Citation missing |
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| |
| Submission date |
Feb 18, 2014 |
| Last update date |
Feb 20, 2014 |
| Contact name |
Maeva Giraudo |
| E-mail(s) |
maeva.giraudo@gmail.com
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| Phone |
514-283-5036
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| Organization name |
Environment Canada
|
| Department |
Water Science and Technology Directorate
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| Lab |
Aquatic Contaminants Research Division
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| Street address |
105 McGill street
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H2Y 2E7 |
| Country |
Canada |
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| Platforms (1) |
| GPL18298 |
Agilent-023710 Daphnia magna (probe name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA238590 |
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