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| Status |
Public on Feb 19, 2014 |
| Title |
The role of Rif1 in ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Purpose:The goals of this study are to understand the mechanisms underlying reduced self-renewal and loss of pluripotency by depletion of Rif1.
Methods: We performed global gene expression analysis of Rif1 knockdown ES cell lines using Affymetrix 430 2.0 arrays, compared to shRNA controls.
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| Overall design |
J1 ES cells were transfected with Control shRNA and Rif1 shRNA using lipofectamine 2000. Forty-eight hours after transfection, cells were collected for global gene expression analysis using Affymetrix mouse genome 430 2.0 array. Stable F1 Rif1 knockdown ES cells at passage 16 were used for long-term gene expression chip microarray using Affymetrix mouse genome 430 2.0 array.
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| Contributor(s) |
Liu L, Liu N |
| Citation(s) |
24735877 |
| |
| Submission date |
Feb 18, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Andrew Zhuo Xiao |
| E-mail(s) |
andrew.xiao@yale.edu
|
| Phone |
203-785-5111
|
| Organization name |
Yale Stem Cell Center
|
| Department |
Genetics
|
| Lab |
Andrew Xiao
|
| Street address |
10 Amistad St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA238588 |
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