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| Status |
Public on Feb 15, 2014 |
| Title |
Small intestine intraepithelial CD4+ T cells after Toxoplasma gondii infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Retention of lymphocytes in the intestinal mucosa requires specialized chemokine receptors and adhesion molecules. Here we find that both CD4+CD8+ and CD4+T cells in the intestinal epithelium, as well as CD8+T cells in the intestinal mucosa and mesenteric lymph nodes, express the cell adhesion molecule Crtam upon activation, whereas the ligand of Crtam, Cadm1, is expressed on gut CD103+DCs. Lack of Crtam-Cadm1 interactions in Crtam-/- and Cadm1-/- mice results in loss of CD4+CD8+T cells, which arise from mucosal CD4+T cells that acquire a CD8 lineage expression profile. Following acute oral infection with T. gondii, both WT and Crtam-/- mice mounted a robust TH1 response, but markedly fewer TH17 cells were present in the intestinal mucosa of Crtam-/- mice. The almost exclusive TH1 response in Crtam-/- mice resulted in more efficient control of intestinal T. gondii infection.
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| Overall design |
CD4+ T cells were cell sorted to analyze the differences resulting from the lack of Crtam expression during T. gondii infection.
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| Contributor(s) |
Colonna M, Cortez VS, Cervantes-Barragan L |
| Citation(s) |
24687959 |
| |
| Submission date |
Feb 14, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Marco Colonna |
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Street address |
660 S Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA238368 |
