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Series GSE54825 Query DataSets for GSE54825
Status Public on Apr 10, 2015
Title Transcriptional reprogramming of engineered cellobiose-utilizing Saccharomyces cerevisiae in response to cellobiose revealed by RNA-Seq
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Saccharomyces cerevisiae cannot metabolize cellobiose in nature. Here, S. cerevisiae was engineered to achieve cellobiose utilization by introducing both a cellodextrin transporter gene (cdt-1) and an intracellular β-glucosidase gene (gh1-1) from Neurospora crassa. We sequenced mRNA from anaerobic exponential cultures of engineered S. cerevisiae grown on cellobiose or glucose as a single carbon source in biological triplicate. Differences in gene expression between cellobiose and glucose metabolism revealed by RNA deep sequencing indicated that cellobiose metabolism induced mitochondrial activation and reduced amino acid biosynthesis under fermentation conditions.
Overall design mRNA levels in cellobiose-grown and glucose-grown cells of engineered cellobiose-utilizing Saccharomyces cerevisiae were examined by deep sequencing, in triplicate, using Illumina Genome Analyzer-II. We sequenced 3 samples from cellobiose-grown cells and 3 samples from glucose-grown cells and identified differential expressions in the cellobiose versus glucose fermentations by using mRNA levels of glucose-grown cells as a reference.
Contributor(s) Lin Y, Cate JH
Citation(s) 25435910
Submission date Feb 10, 2014
Last update date May 15, 2019
Contact name Yuping Lin
Organization name Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
Street address 32 West 7th Avenue, Tianjin Airport Economic Area
City Tianjin
State/province Tianjin
ZIP/Postal code 300308
Country China
Platforms (1)
GPL9377 Illumina Genome Analyzer II (Saccharomyces cerevisiae)
Samples (6)
GSM1324496 JCYL001B
GSM1324497 JCYL002B
GSM1324498 JCYL003B
BioProject PRJNA237759
SRA SRP037533

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Supplementary file Size Download File type/resource
GSE54825_Cellobiose_versus_Glucose.xlsx.gz 708.7 Kb (ftp)(http) XLSX
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