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| Status |
Public on Apr 10, 2015 |
| Title |
Transcriptional reprogramming of engineered cellobiose-utilizing Saccharomyces cerevisiae in response to cellobiose revealed by RNA-Seq |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Saccharomyces cerevisiae cannot metabolize cellobiose in nature. Here, S. cerevisiae was engineered to achieve cellobiose utilization by introducing both a cellodextrin transporter gene (cdt-1) and an intracellular β-glucosidase gene (gh1-1) from Neurospora crassa. We sequenced mRNA from anaerobic exponential cultures of engineered S. cerevisiae grown on cellobiose or glucose as a single carbon source in biological triplicate. Differences in gene expression between cellobiose and glucose metabolism revealed by RNA deep sequencing indicated that cellobiose metabolism induced mitochondrial activation and reduced amino acid biosynthesis under fermentation conditions.
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| Overall design |
mRNA levels in cellobiose-grown and glucose-grown cells of engineered cellobiose-utilizing Saccharomyces cerevisiae were examined by deep sequencing, in triplicate, using Illumina Genome Analyzer-II. We sequenced 3 samples from cellobiose-grown cells and 3 samples from glucose-grown cells and identified differential expressions in the cellobiose versus glucose fermentations by using mRNA levels of glucose-grown cells as a reference.
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| Contributor(s) |
Lin Y, Cate JH |
| Citation(s) |
25435910 |
| |
| Submission date |
Feb 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuping Lin |
| E-mail(s) |
lin_yp@tib.cas.cn
|
| Organization name |
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
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| Street address |
32 West 7th Avenue, Tianjin Airport Economic Area
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300308 |
| Country |
China |
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| Platforms (1) |
| GPL9377 |
Illumina Genome Analyzer II (Saccharomyces cerevisiae) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA237759 |
| SRA |
SRP037533 |
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