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| Status |
Public on Dec 31, 2014 |
| Title |
Induced CD103+ Dendritic Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Multiple subsets of FLT3L-dependent dendritic cells (DCs) control T cell tolerance and immunity. In mouse tissues, CD8α-like DCs are identified by CD103 expression. This DC subset efficiently enters lymph nodes and cross-presents antigens, rendering CD103+ DCs promising targets for therapeutic tolerance induction or vaccination. However, only limited numbers of CD103+ DCs can be isolated with current methods. Moreover, bone marrow cultures with FLT3L produce complex mixtures of DC subsets. We developed a novel method for generating large numbers of Batf3-dependent CD103+ DCs.
We used microarray analysis to compare in vitro generated CD103+ and CD103- DCs and correlated their expression patterns to published profiles and signatures of DC subsets.
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| Overall design |
We generated iCD103 DCs according to our new method. We sorted CD11c+B220-CD103hi and CD11c+B220-CD103- DC subsets and processed these populations for RNA extraction and hybridization to Agilent microarrays.
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| Contributor(s) |
Mayer CT, Sparwasser T |
| Citation(s) |
25100743 |
| |
| Submission date |
Jan 28, 2014 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
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| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA236596 |
