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Series GSE54472 Query DataSets for GSE54472
Status Public on Dec 31, 2014
Title Induced CD103+ Dendritic Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Multiple subsets of FLT3L-dependent dendritic cells (DCs) control T cell tolerance and immunity. In mouse tissues, CD8α-like DCs are identified by CD103 expression. This DC subset efficiently enters lymph nodes and cross-presents antigens, rendering CD103+ DCs promising targets for therapeutic tolerance induction or vaccination. However, only limited numbers of CD103+ DCs can be isolated with current methods. Moreover, bone marrow cultures with FLT3L produce complex mixtures of DC subsets. We developed a novel method for generating large numbers of Batf3-dependent CD103+ DCs.
We used microarray analysis to compare in vitro generated CD103+ and CD103- DCs and correlated their expression patterns to published profiles and signatures of DC subsets.
Overall design We generated iCD103 DCs according to our new method. We sorted CD11c+B220-CD103hi and CD11c+B220-CD103- DC subsets and processed these populations for RNA extraction and hybridization to Agilent microarrays.
Contributor(s) Mayer CT, Sparwasser T
Citation(s) 25100743
Submission date Jan 28, 2014
Last update date Nov 01, 2017
Contact name Robert Geffers
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (2)
GSM1316343 iCD103 CD103+
GSM1316344 iCD103 CD103-
BioProject PRJNA236596

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54472_RAW.tar 4.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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