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| Status |
Public on Jan 13, 2017 |
| Title |
Hypoxia-inducible factor 3 is an oxygen-dependent transcription activator and regulates a distinct transcriptional response to hypoxia |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
This study aimed to identify Hif-1 and -3 regulated genes in zebrafish embryos. RNA samples were prepared from embryos injected with GFP, Hif-1α, and Hif-3α mRNA. The RNA samples were subjected to DNA microarray analysis using the Agilent 4×44 K (V3) Zebrafish Microarray Chip.
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| Overall design |
Three biological replicates were collected from three independent microinjection experiments. Each RNA sample was extracted from a pool of 60 embryos and labeled with Cy3-CTP. Slides were scanned by the Agilent DNA Microarray Scanner, read by Feature Extraction software 10.7 and normalized by Gene Spring Software 11.0.
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| Contributor(s) |
Zhang P, Duan C |
| Citation(s) |
24613356 |
| |
| Submission date |
Jan 22, 2014 |
| Last update date |
Jan 13, 2017 |
| Contact name |
Peng Zhang |
| Organization name |
University of Michigan
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| Department |
Molecular, Cellular, and Developmental Biology
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| Lab |
Duan Lab
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| Street address |
830 N. University Ave.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL14664 |
Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA236310 |
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