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| Status |
Public on Jan 23, 2015 |
| Title |
Expression data of human fetal liver hematopoietic stem and progenitors cells [Set 1] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Advances in pluripotent stem cell and reprogramming technologies have given hope of generating hematopoietic stem cells (HSC) in culture. To succeed, greater understanding of the self-renewing HSC during human development is required. We discovered that glycophosphatidylinositol-anchored surface protein GPI-80 (Vanin 2) defines a distinct subpopulation of human fetal hematopoietic stem/progenitor cells (HSPC) with self-renewal ability. CD34+CD90+CD38-GPI-80+ HSPC were the sole population that maintained proliferative potential and undifferentiated state in bone marrow stroma co-culture, and engrafted in immunodeficient mice. GPI-80 expression also enabled tracking of HSC migration between human fetal hematopoietic niches. The most highly enriched surface protein in GPI-80+ HSPC as compared to their progeny was Integrin alpha-M (ITGAM), which in leukocytes cooperates with GPI-80 to support migration. Knockdown of either GPI-80 or ITGAM was sufficient to perturb undifferentiated HSPC in stroma co-culture. These findings indicate that human fetal HSC utilize common mechanisms with leukocytes for cell-cell interactions governing HSC self-renewal.
We used microarrays to identify genes enriched in the CD90+ hematopoietic stem and progenitor population in fetal liver.
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| Overall design |
RNA was extracted from enriched fetal liver hematopoietic stem and progenitor cells, and downstream progenitors, for comparison based on Affymetrix arrays.
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| Contributor(s) |
Prashad S, Yao C, Calvanese V, Kaiser J, Sasidharan R, Magnusson M, Mikkola H |
| Citation(s) |
25465114 |
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| Submission date |
Jan 22, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Hanna K.A. Mikkola |
| Organization name |
UCLA
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| Department |
MCDB
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| Street address |
615 Charles E Young Drive South
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| City |
Los Angeles, CA |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (7)
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| GSM1312830 |
human fetal liver cells, hematopoietic stem cell enriched, rep 1 |
| GSM1312831 |
human fetal liver cells, hematopoietic stem cell enriched, rep 2 |
| GSM1312832 |
human fetal liver cells, hematopoietic stem cell enriched, rep 3 |
| GSM1312833 |
human fetal liver cells, hematopoietic progenitor enriched, rep 1 |
| GSM1312834 |
human fetal liver cells, hematopoietic progenitor enriched, rep 2 |
| GSM1312835 |
human fetal liver cells, hematopoietic progenitor enriched, rep 3 |
| GSM1312836 |
human fetal liver cells, hematopoietic progenitor enriched, rep 4 |
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| This SubSeries is part of SuperSeries: |
| GSE54316 |
Expression data of human fetal liver hematopoietic stem and progenitors cells |
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| Relations |
| BioProject |
PRJNA236142 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE54314_RAW.tar |
30.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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