|
Status |
Public on Mar 01, 2014 |
Title |
Tissue culture model of hypothermia |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Therapeutic hypothermia is a clinically effective treatment for various hypoxic and ischemic conditions, but the associated molecular mechanisms remain unclear. To gain insight into hypothermia-induced transcriptional response, mouse embryonic fibroblasts were exposed to mild hypothermia (32°C) or normothermia (37°C) for increasing time periods. We aimed to identify genes with temporally near-monotonic response as the most obvious candidates for mediating the therapeutic effects of hypothermia.
|
|
|
Overall design |
Hypothermia was compared against normothermia at seven different time-points. Hypothermic and normothermic mouse embryonic fibroblasts (MEF, in 100 mm dishes, one array per time-point) were submitted to gene expression analysis using Mouse Gene 1.0 ST GeneChip® (Affymetrix) microarrays. Differential gene expression analysis was performed with R package DEMI (http://biit.cs.ut.ee/demi/).
|
|
|
Contributor(s) |
Ilmjärv S, Luuk H, Hundhal CA, Reimets R, Niitsoo M, Kolde R, Vilo J, Vasar E |
Citation(s) |
24586062 |
|
Submission date |
Jan 21, 2014 |
Last update date |
Mar 04, 2019 |
Contact name |
Sten Ilmjärv |
E-mail(s) |
sten.ilmjarv@gmail.com
|
Organization name |
University of Tartu
|
Department |
Institute of Biomedicine and Translational Medicine
|
Lab |
Department of Physiology
|
Street address |
Ravila 19
|
City |
Tartu |
State/province |
Tartumaa |
ZIP/Postal code |
50411 |
Country |
Estonia |
|
|
Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
Samples (13)
|
|
Relations |
BioProject |
PRJNA235997 |