|
| Status |
Public on Dec 31, 2014 |
| Title |
Genome wide analysis of the direct and indirect consequences on gene expression of a thyroid hormone receptor alpha 1 mutation restricted to Sertoli cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A dominant negative allele of TRα1 (TRαAMI) has been introduced only in Sertoli cells using the Cre/lx system and the AmhCre mouse line. Testes were isolated at P3 and RNA was extracted. MRNA were enriched by oligo-dT purification and submitted to Illumina HiSeq2000 sequencing. Reads were aligned on the mouse mm9 genome, gene counts were obtained with the HTSeq-count script and differential expression was investigated with the DESeq2 R package.
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| |
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| Overall design |
Examination of thyroid hormone target genes in testes after disruption of the receptor in only the Sertoli cells.
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| |
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| Contributor(s) |
Chatonnet F, Fouchecourt S, Flamant F |
| Citation(s) |
25487743 |
| |
| Submission date |
Jan 15, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Fabrice Chatonnet |
| E-mail(s) |
fabrice.chatonnet@ens-lyon.org
|
| Phone |
+33 2 99 28 99 39
|
| Organization name |
University Hospital of Rennes
|
| Lab |
Inserm U1236 MICMAC
|
| Street address |
2 rue Henri le Guilloux
|
| City |
Rennes |
| ZIP/Postal code |
35033 |
| Country |
France |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA235200 |
| SRA |
SRP035418 |
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