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| Status |
Public on Apr 06, 2017 |
| Title |
DFO-induced hypoxia leading to metabolic dysfunction in WAT |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Metabolic dysfunction of white adipose tissue (WAT) is considered to be underlying the comorbidities in obesity, including insulin resistance and tissue inflammation. Moreover, due to the expansion of WAT, local tissue hypoxia has been reported. Whether local tissue hypoxia underlies the metabolic de-arrangements and is the first step in initiation inflammation is questioned here. Desferrioxamine (DFO) is a chemical compound trapping free iron, thus leading to a reduction in oxygen availability within the body, which mimics hypoxia. Therefore, C57BL/6JOlaHsd wildtype male mice, aged 9 weeks, were fed purified low fat diet (BIOCLAIMS, Hoevenaars et al., Genes and Nutrition 2012) for 3 weeks to acclimatize, followed by 12 weeks a BIOCLAIMS high-fat diet (HFD), all at thermoneutrality (29 degrees C) to induce massive expansion of WAT without metabolic dysregulation (Hoevenaars et al., Mol Nutr Food Res 2014). Subsequently, mice were divided into different treatment groups: i) control group, ii) 5 days Desferrioxamine (DFO) daily injections (100 mg/kg body weight). Mice were killed by decapitation at the end of the experiment after 2 hour food removal at the start of the light phase. After sacrification, epididymal WAT was immediately dissected and snap frozen in liquid nitrogen. Total RNA was isolated, quantified and qualified, and subsequently used for global gene expression profiling using Agilent 8x60K microarrays.
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| Overall design |
C57BL/6JOlaHsd wildtype male mice, aged 9 weeks, were fed purified low fat diet (BIOCLAIMS, Hoevenaars et al., Genes and Nutrition 2012) for 3 weeks to acclimatize, followed by 12 weeks a BIOCLAIMS high-fat diet (HFD), all at thermoneutrality (29 degrees C). Subsequently, mice were divided into different treatment groups: i) control group, ii) 5 days Desferrioxamine (DFO) daily injections (100 mg/kg body weight). Mice were killed by decapitation at the end of the experiment after 2 hour food removal at the start of the light phase. After sacrification, epididymal WAT was immediately dissected and snap frozen in liquid nitrogen. Total RNA was isolated, quantified and qualified, and subsequently used for global gene expression profiling using Agilent 8x60K microarrays.
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| Contributor(s) |
Hoevenaars F, van Schothorst E, van der Stelt I, Keijer J |
| Citation(s) |
28386236 |
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| Submission date |
Jan 03, 2014 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Evert M. van Schothorst |
| E-mail(s) |
evert.vanschothorst@wur.nl
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| Organization name |
Wageningen University
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| Lab |
Human and Animal Physiology
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| Street address |
De Elst 1
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| City |
Wageningen |
| ZIP/Postal code |
6708 WD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (10)
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| GSM1301081 |
HFD, DFO injected, replicate 4 |
| GSM1301082 |
HFD, DFO injected, replicate 5 |
| GSM1301083 |
HFD, DFO injected, replicate 6 |
| GSM1301084 |
HFD, DFO injected, replicate 7 |
| GSM1301085 |
HFD, DFO injected, replicate 8 |
| GSM1301086 |
HFD, DFO injected, replicate 9 |
| GSM1301087 |
HFD, DFO injected, replicate 10 |
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| This SubSeries is part of SuperSeries: |
| GSE53805 |
External factors inducing metabolic adaptations in white adipose tissue in wildtype C57BL/6J mice housed at thermoneutrality. |
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| Relations |
| BioProject |
PRJNA233387 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53803_RAW.tar |
215.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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