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| Status |
Public on Dec 21, 2013 |
| Title |
RNA expression data from Halobacterium NRC-1 in varied extracellular salinity conditions |
| Organism |
Halobacterium salinarum NRC-1 |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
Halobacterium salinarum is an extreme halophilic archaeon adapted to total salinities upwards of 4 M. Here we studied gene expression in H. salinarum grown in ten ion composition media that vary in two major aspects of ion composition: [NaCl] and Mg:K ratio.
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| Overall design |
Samples were taken from the Δura3 parental strain cells at mid-log phase (OD600 = 0.4-0.5) during growth in ten different media. RNA from growth in each media was compared with a standard RNA reference, extracted from H. salinarum NRC-1 grown in batch culture in the standard growth media to OD = 0.471. Two biological replicates were performed for each media, with the exception of one replicate only for M12.
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| Contributor(s) |
Beer KD, Wurtmann EJ, Pinel N, Baliga NS |
| Citation(s) |
24413600, 25806405 |
| |
| Submission date |
Dec 20, 2013 |
| Last update date |
Jun 07, 2019 |
| Contact name |
Nicolas Pinel |
| E-mail(s) |
pinel.n@gmail.com
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| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Ave N
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL17005 |
Agilent-032722 Halobacterium sp. NRC-1 Tiling V2 030521 |
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| Samples (20)
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| GSM1295943 |
Δura3 Media M6 biological replicate 2 |
| GSM1295944 |
Δura3 Media M7 biological replicate 2 |
| GSM1295945 |
Δura3 Media M9 biological replicate 2 |
| GSM1295946 |
Δura3 Media M12 biological replicate 2 |
| GSM1295947 |
Δura3 Media M13 biological replicate 2 |
| GSM1295948 |
Δura3 Media M14 biological replicate 2 |
| GSM1295949 |
Δura3 Media M15 biological replicate 2 |
| GSM1295950 |
Δura3 Media M2 biological replicate 1 |
| GSM1295951 |
Δura3 Media M6 biological replicate 1 |
| GSM1295952 |
Δura3 Media M1 biological replicate 1 |
| GSM1295953 |
Δura3 Media M2 biological replicate 1 technical replicate 2 |
| GSM1295954 |
Δura3 Media M5 biological replicate 1 |
| GSM1295955 |
Δura3 Media M7 biological replicate 1 |
| GSM1295956 |
Δura3 Media M9 biological replicate 1 |
| GSM1295957 |
Δura3 Media M13 biological replicate 1 |
| GSM1295958 |
Δura3 Media M14 biological replicate 1 |
| GSM1295959 |
Δura3 Media M15 biological replicate 1 |
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| Relations |
| BioProject |
PRJNA232350 |
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