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| Status |
Public on Feb 01, 2014 |
| Title |
Genome-wide map of RNA polymerase II, NELF, and DSIF in HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Most metazoan promoters have RNA polymerase II (Pol II) paused slightly downstream of the transcription start site by NELF and DSIF. This pausing keeps these promoters available for rapid induction by P-TEFb, whose activity causes NELF to dissociate and Pol II and DSIF to elongate on the gene. ChIP-Seq data was generated for Pol II, NELF, and DSIF in HeLa cells and used to look at pausing downstream of unannotated promoters.
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| Overall design |
3x ChIP-Seq (Pol II, NELF, DSIF) in HeLa cells
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| Contributor(s) |
Nilson KA, Price DH |
| Citation(s) |
24515107 |
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| Submission date |
Dec 05, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
David Price |
| E-mail(s) |
david-price@uiowa.edu
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| Organization name |
University of Iowa
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| Department |
Biochemistry
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| Lab |
260 EMRB
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| Street address |
500 Newton Road
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| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA230673 |
| SRA |
SRP033525 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53008_RAW.tar |
263.6 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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