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| Status |
Public on Nov 14, 2013 |
| Title |
Expression data from peritoneal macrophages stimulated with trehalose-6,6'-dimycolate (TDM) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Macrophage-inducible C-type lectin (Mincle, Clec4e) is a pathogen sensor that recognizes pathogenic fungi and Mycobactrium tuberculosis. We perfomed microarray analysis using peritoneal macrophages stimulated with TDM, a mycobacterial cell wall glycolipid that is known to be a Mincle ligand.
Many chemokine and cytokine genes were upregulated in wildtype macrophages stimulated with TDM. Upregulation of these genes were completely abolishd in Mincle KO macrophages.
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| Overall design |
Peritoneal macrophages from WT and Mincle KO mice were stimulated with TDM or vehicle for 24 h (3 samples each). Microarray analysis was performed using Affymetrix Mouse 430 2.0.
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| Contributor(s) |
Komiya C, Matsuda T, Tanaka M, Suganami T, Ogawa Y |
| Citation(s) |
25236782 |
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| Submission date |
Nov 13, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Chikara Komiya |
| E-mail(s) |
ckomiya.mem@tmd.ac.jp
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| Organization name |
Tokyo Medical and Dental University
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| Department |
Molecular Endocrinology and Metabolism
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| Street address |
1-5-45 Yushima
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| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1138510 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM1263330 |
KO, vehicle, 1 |
| GSM1263331 |
KO, vehicle, 2 |
| GSM1263332 |
KO, vehicle, 3 |
| GSM1263333 |
WT, TDM, 1 |
| GSM1263334 |
WT, TDM, 2 |
| GSM1263335 |
WT, TDM, 3 |
| GSM1263336 |
KO, TDM, 1 |
| GSM1263337 |
KO, TDM, 2 |
| GSM1263338 |
KO, TDM, 3 |
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| Relations |
| BioProject |
PRJNA227446 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52338_RAW.tar |
200.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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