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Series GSE52124 Query DataSets for GSE52124
Status Public on Mar 07, 2014
Title TAp73 is essential for germ cell adhesion and maturation in testis
Organism Mus musculus
Experiment type Expression profiling by array
Summary A core evolutionary function of the p53 family is to protect the genomic integrity of gametes. However, the role of p73 in the male germline is unknown. Here we uncover that TAp73 unexpectedly functions as adhesion and maturation factor of the seminiferous epithelium orchestrating spermiogenesis. TAp73KO and p73KO mice, but not ΔNp73KO mice, display a ‘near-empty seminiferous tubule’ phenotype due to massive premature loss of immature germ cells. Its cellular basis are defective cell-cell adhesions of developing germ cells to Sertoli nurse cells, with secondary degeneration of Sertoli cells including the blood-testis-barrier, thereby disrupting the adhesive integrity and maturation of the germ epithelium. At the molecular level, TAp73, produced in germ cells, controls a coordinated transcriptional program of adhesion- and migration-related proteins including peptidase inhibitors, proteases, receptors and integrins required for germ-Sertoli cell adhesion and dynamic junctional restructuring. Thus, the testis emerges as unique organ with strict division of labor among all family members: p63 and p53 safeguard germline fidelity, while TAp73 ensures fertility by enabling sperm maturation.
 
Overall design 3 mice each for control wildtype and TAp73 knockout mice
 
Contributor(s) Holembowski L, Kramer D, Riedel D, Sordella R, Dobbelstein M, Moll UM, Nemajerova A
Citation(s) 24662569
Submission date Nov 06, 2013
Last update date Nov 01, 2017
Contact name Gabriela Salinas
E-mail(s) Gabriela.Salinas-Riester@medizin.uni-goettingen.de
Organization name Universitaetsmedizin Goettingen
Department Department of Pathology
Lab NGS Integrative Genomics
Street address Kreuzbergring 57
City Goettingen
State/province Lower-Saxony
ZIP/Postal code 37075
Country Germany
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (12)
GSM1260043 120KO SERTOLI CELLS
GSM1260044 86KO SERTOLI CELLS
GSM1260045 72KO SERTOLI CELLS
Relations
BioProject PRJNA226753

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52124_RAW.tar 26.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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