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Series GSE52070 Query DataSets for GSE52070
Status Public on Mar 05, 2014
Title Genome-wide maps of Tcf1 binding locations in splenic CD8 T cells.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We mapped genome-wide Tcf1 binding locations in mature CD8 T cells to identify its direct target genes.
Overall design This experiment aims to identify genome-wide binding locations for Tcf1 transcription factor in mature CD8 T cells. The CD8 T cells were isolated from wild-type C57BL/6 mice by negative selection. The chromatin fragments were immunoprecipitated by an anti-Tcf1 antiserum or control rabbit serum (without immunization). The immunoprecipitated fragments were then used in high throughput sequencing. Data processing then revealed >3000 high-confidence Tcf1 binding peaks across the CD8 T cell genome. One important finding is that Tcf1 binds directly to Cd4 gene silencer, and thus explain its important role in suppressing the CD4 coreceptor in mature CD8 T cells.
Contributor(s) Hai-Hui X, Kai T
Citation(s) 24836425
Submission date Nov 04, 2013
Last update date May 15, 2019
Contact name Hai-Hui Xue
Organization name Hackensack University Medical Center
Department Center for Discovery and Innovation
Street address 111 Ideation Way
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM1258235 Tcf1-ChIPSeq
GSM1258236 IgG-ChIPSeq
BioProject PRJNA226615
SRA SRP032529

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Supplementary file Size Download File type/resource
GSE52070_Tcf1_peaks.bed.gz 30.9 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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