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| Status |
Public on Mar 05, 2014 |
| Title |
Genome-wide maps of Tcf1 binding locations in splenic CD8 T cells. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We mapped genome-wide Tcf1 binding locations in mature CD8 T cells to identify its direct target genes.
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| Overall design |
This experiment aims to identify genome-wide binding locations for Tcf1 transcription factor in mature CD8 T cells. The CD8 T cells were isolated from wild-type C57BL/6 mice by negative selection. The chromatin fragments were immunoprecipitated by an anti-Tcf1 antiserum or control rabbit serum (without immunization). The immunoprecipitated fragments were then used in high throughput sequencing. Data processing then revealed >3000 high-confidence Tcf1 binding peaks across the CD8 T cell genome. One important finding is that Tcf1 binds directly to Cd4 gene silencer, and thus explain its important role in suppressing the CD4 coreceptor in mature CD8 T cells.
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| Contributor(s) |
Hai-Hui X, Kai T |
| Citation(s) |
24836425 |
| Submission date |
Nov 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Hai-Hui Xue |
| E-mail(s) |
haihui.xue@hmh-cdi.org
|
| Organization name |
Hackensack University Medical Center
|
| Department |
Center for Discovery and Innovation
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| Street address |
111 Ideation Way
|
| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA226615 |
| SRA |
SRP032529 |
