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Series GSE52043 Query DataSets for GSE52043
Status Public on Apr 26, 2014
Title Tissue-Resident Natural Killer (NK) Cells Are Cell Lineages Distinct From Thymic and Conventional Splenic NK Cells (part 1)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Natural killer (NK) cells belong to the innate immune system where they can control virus infections and developing tumors by cytotoxicity and production of inflammatory cytokines. Most studies of mouse NK cells, however, have focused on conventional NK (cNK) cells found in the spleen. Recently, we described two populations of NK cells within the liver, tissue-resident NK (trNK) cells and those resembling splenic cNK cells. However, the lineage relationship of trNK to cNK cells was unclear because trNK cells display a phenotype associated with immature, developing cNK cells. Moreover, liver trNK cells could be related to thymic NK cells or alternatively, a lineage distinct from both cNK and thymic NK cells. Herein we used detailed transcriptomic, flow cytometric, and functional analysis of mice deficient in several transcription factors to determine that liver trNK cells form a distinct lineage from cNK and thymic NK cells, especially because they do not require NFIL3 (E4BP4), the previously described NK cellspecification factor. Analysis of other tissues indicate the presence of trNK cells in skin and uterus with different transcription factor requirements. Thus, there are at least four distinct lineages of NK cells: cNK, thymic, liver (and skin) trNK, and uterine trNK cells.
Overall design Liver NK 1.1+CD49+, liver NK 1.1+CD49-, spleen NK 1.1+ CD49- populations of NK cells were sorted with FACS pooling cells from individual mice to end up with ~100k cells for each samples. mRNA was derived from lysates using Invitrogen oligo-dT beads
Contributor(s) Artyomov MN, Ivanova Y
Citation(s) 24714492
Submission date Nov 04, 2013
Last update date May 15, 2019
Contact name Maxim N. Artyomov
Organization name Washington University in St.Louis
Department Immunology&Pathology
Street address 660 S. Euclid Avenue, Campus Box 8118
City St.Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM1257948 Liver NK1.1+ CD49+ rep1
GSM1257949 Liver NK1.1+ CD49+ rep2
GSM1257950 Liver NK1.1+ CD49- rep1
This SubSeries is part of SuperSeries:
GSE52047 Tissue-Resident Natural Killer (NK) Cells Are Cell Lineages Distinct From Thymic and Conventional Splenic NK Cells
BioProject PRJNA226577
SRA SRP032515

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Supplementary file Size Download File type/resource
GSE52043_Barcodes.txt.gz 150 b (ftp)(http) TXT
GSE52043_NK_Set1.norm.nodup.annot.log2.coll.txt.gz 841.3 Kb (ftp)(http) TXT
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Processed data are available on Series record

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