|
| Status |
Public on Nov 12, 2013 |
| Title |
Targeted degradation of sense and antisense C9orf72 RNA foci as therapy for amyotrophic lateral sclerosis and frontotemporal dementia (strand specific RNA-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The purpose of this experiment is to identify expression changes after ASO-dependent depletion of mouse C9orf72 in the spinal cord of wild-type C57Bl/6 female mice.
Methods: Strand specific RNA-seq was performed using RNAs extracted from spinal cord of C57Bl/6 mice two weeks after intracerebroventricular stereotactic injection of saline (n=3), a control ASO (n=3) or an ASO targeting mouse C9orf72 (n=3). C9orf72 RNA levels were reduced to approximately 30% of control levels in spinal cords from mice treated with the C9orf72 ASO.
Results: Statistical comparison of RPKM values between RNAs from C9orf72 and control ASO treated animals or C9orf72 and saline treated samples revealed that only 12 genes were consistently upregulated (defined by P<0.05 adjusted for multiple testing) and 12 genes including C9orf72 were downregulated (defined by P<0.05 adjusted for multiple testing).
Conclusions: Only few RNA expression changes were identified in the spinal cord following reduction of C9orf72.
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| |
|
| Overall design |
Use of strand specific RNA-seq to test the consequences of C9orf72 loss of function in mouse spinal cord.
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| |
|
| Contributor(s) |
Lagier-Tourenne C, Baughn M, Rigo F, Liu P, Hutt K, Yeo GW, Cleveland D, Ravits J |
| Citation(s) |
24170860 |
| |
| Submission date |
Oct 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
John Ravits |
| E-mail(s) |
jravits@ucsd.edu
|
| Organization name |
UCSD
|
| Department |
Neuroscience
|
| Lab |
Ravits
|
| Street address |
9500 Gilman Dr. #0624
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0624 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (9)
|
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| This SubSeries is part of SuperSeries: |
| GSE51741 |
Targeted degradation of sense and antisense C9orf72 RNA foci as therapy for amyotrophic lateral sclerosis and frontotemporal dementia |
|
| Relations |
| BioProject |
PRJNA224741 |
| SRA |
SRP032164 |
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