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Series GSE51284 Query DataSets for GSE51284
Status Public on Sep 30, 2015
Title Expression data from monozygotic twins discordant for nicotine metabolism
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The Nicotine Metabolic Ratio (NMR), the ratio of two stable metabolites of nicotine, cotinine and trans-3’hydroxycotinin, is a determinant of the number of cigarettes smoked per day (CPD), of smoking topography, responsiveness to treatment via transdermal nicotine for smoking cessation, and carcinogen activation and level. The predominant gene involved in nicotine metabolism is CYP2A6, a highly polymorphic locus located in a cluster of P450 cytochrome protein loci on chr19q13. A recent study comprehensively modeling CYP2A6 genetic variation at seven polymorphisms and a related Nicotine Metabolic Ratio, the cotinine: cotinine+nicotine ratio, explains 72% of NMRC/C+N variation. These results suggest that additional genes may be contributing to nicotine and cotinine metabolism directly or via interaction with CYP2A6 or other genes. To identify additional genes that may influence the NMR, we conducted a genome-wide gene expression analysis of lymphoblastoid cell line (LCL) gene expression in a sample of monozygotic twins discordant for the NMR.
Overall design Eight individuals (four monozygotic twin pairs), each with three replicates for a total of 24 samples
Contributor(s) Bergen AW, Wacholder AC, Nishita DM, Michel M, Krasnow R, Javitz HS, Fugman DA, Tischfield JA, Hops H, Benowitz NL, Enoch M, Swan GE
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Submission date Sep 30, 2013
Last update date Nov 08, 2016
Contact name Aaron Wacholder
Organization name SRI International
Department Molecular Genetics Program
Street address 333 Ravenswood Ave
City Menlo Park
State/province CA
ZIP/Postal code 94025
Country USA
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (24)
GSM1241832 LCL_highNMR_pair10370_rep1
GSM1241833 LCL_highNMR_pair10242_rep1
GSM1241834 LCL_lowNMR_pair10370_rep1
BioProject PRJNA222305

Download family Format
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Supplementary file Size Download File type/resource
GSE51284_RAW.tar 98.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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