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| Status |
Public on Sep 12, 2013 |
| Title |
Cyclophilin B supports the survuval of glioblastoma multiforme cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have found that cyclophilin B (CypB) expression is important for malignant glioblastoma multiforme (GBM) cell proliferation. To identify molecular mechanisms that could explain CypB-dependent survival in human GBM cells, a microarray analysis was performed using RNA prepared from U251MG GBM cells transduced with lentiviral CypB shRNA. These data revealed that about 130 genes were more than 2-fold affected by CypB depletion. Significant alterations in the expression of genes related to cell death, cell proliferation and cell migration were found in the shCypB cells.
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| Overall design |
U251 glioblastoma cells transduced with lentivirus expressing non-target control shRNA (shCON) were compared to cells transduced with lentivirus expressing shRNA sequence against cyclophilin B (shCypB). Cells transduced with the lentiviral shRNA (shCON VS shCypB) for 5 days before total RNA extraction. Three biological replicates of cells treated with each shRNA (shCON or shCypB) were profiled.
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| Contributor(s) |
Bram RJ, Choi JW |
| Citation(s) |
24272483 |
| |
| Submission date |
Sep 11, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Richard J Bram |
| E-mail(s) |
richard.bram@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Pediatric and Adolescent Medicine
|
| Street address |
200 First Street SW
|
| City |
ROCHESTER |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA218845 |
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