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| Status |
Public on Sep 07, 2013 |
| Title |
Genome-wide transcription profile of CpG-activated peritoneal macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To compare up-regulation of genes following CpG activation, we performed microarray analysis of activated macrophages from B6 and F1(B6xMOLF) mouse strains. Cells were activated for 0, 2 and 4 hrs with 200nM of type B CpG. Levels of mRNA for many genes differened dramatically between the strains
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| Overall design |
Peritoneal macrophages were elicited from pertoneal cavity of mice 3 days after thioglycollate injection. The cells were plated overnight at a density of 5 million cells per 100 mm dish and activated the next day with 200 nM CpG. Total RNA was isolated with TRIZOL followed by reverse transcrition, fragmentation and labeling accroding the manufacture's (Affymetrix) recommendations
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| Contributor(s) |
Moseman A, Poltorak A |
| Citation(s) |
24184555, 26685207 |
| |
| Submission date |
Sep 06, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Alexander Poltorak |
| Phone |
6176363596
|
| Organization name |
Tufts University
|
| Street address |
150 Harrison avenue
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM1226228 |
Non-activated C57BL6 macrophages |
| GSM1226229 |
CpG-activated for 2 hrs C57BL6 macrophages |
| GSM1226230 |
CpG-activated for 4 hrs C57BL6 macrophages |
|
| Relations |
| BioProject |
PRJNA218465 |
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