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Series GSE50660 Query DataSets for GSE50660
Status Public on Nov 25, 2014
Title Cigarette Smoking Reduces DNA Methylation Levels at Multiple Genomic Loci but the Effect is Partially Reversible upon Cessation
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary DNA methylation is an epigenetic event whose pattern is altered frequently in a wide variety of human diseases. Smoking affects DNA methylation possibly leading to abnormal expression of a broad spectrum of genes which in turn may result to the various side effects and diseases associated with smoking. The long term effects of smoking have been widely studied but the mechanism(s) by which those effects may be reversible by smoking cessation are not clearly understood. Here, we conducted an epigenome-wide association study in peripheral-blood DNA in 464 individuals who were current, former and never-smokers. We identified 15 distinct loci (10 of which were novel) where DNA methylation was reduced in smokers and was reversed (but did not reach non-smoking levels) upon smoking cessation within 12 weeks. Although the functional impact of this reversal of DNA methylation is still not understood, this study illustrates the potential of epigenomics to provide insights into mechanisms of environmental and lifestyle exposures, and to suggest new avenues for clinical intervention
Overall design Bisulfite converted DNA from the 464 samples were hybridized to the Illumina Infinium HumanMethylation450 BeadChip

Contributor(s) Tsaprouni LG, Yang TP, Bell JT, Dick KJ, Kanoni S, Nisbet J, Viñuela A, Grundberg E, Nelson CP, Meduri E, Buil A, Cambien F, Hengstenberg C, Erdmann J, Schunkert H, Goodall AH, Ouwehand WH, Dermitzakis ET, Spector TD, Samani NJ, Deloukas P
Citation(s) 25424692
Submission date Sep 06, 2013
Last update date Mar 22, 2019
Contact name Tsun-Po Yang
Organization name Wellcome Trust Sanger Institute
Department Human Genetics
Lab Genetics of Complex Traits in Humans
Street address Wellcome Trust Genome Campus
City Cambridge
ZIP/Postal code CB10 1SA
Country United Kingdom
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (464)
GSM1225377 Peripheral blood from CXMA-01-001
GSM1225378 Peripheral blood from CXMA-01-002
GSM1225379 Peripheral blood from CXMA-01-003
BioProject PRJNA218212

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50660_RAW.tar 183.1 Mb (http)(custom) TAR
GSE50660_matrix_processed.txt.gz 575.7 Mb (ftp)(http) TXT
GSE50660_matrix_signal.txt.gz 986.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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