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Series GSE506 Query DataSets for GSE506
Status Public on Jul 16, 2003
Title Fibroblast cells immediate-early after human cytomegalovirus infection
Organism Homo sapiens
Experiment type Expression profiling by SAGE
Summary Human cytomegalovirus (HCMV) has been shown to have the potential to alter cellular gene expression early after infection. However, one-gene approaches and the use of closed system gene expression technologies have identified only few cellular genes whose activity changed immediate-early. We therefore used serial analysis of gene expression (SAGE) to investigate the transcriptional program of human fibroblasts in response to HCMV in the immediate-early phase of infection. Differential expression of various cellular genes was monitored. Transcriptional expression changes of genes coding for ribosomal proteins reflected a general cellular response to starvation and stress. But differential regulation of genes coding for transcription factors and proteins associated with cellular metabolism, homeostasis and cell structure may represent transcriptional alterations in response to HCMV infection. Expression kinetics by 5' nuclease fluorigenic real-time PCR of selected genes revealed partial protection of infected cells against initial stress-associated alterations of gene expression and indicated fluctuations of transcriptional levels over time. Additionally, agreement with the quantitative results obtained by SAGE was observed only for genes up-regulated in HCMV-infected cells. This finding pointed to various technical and statistical parameters that all may be critical for quantitative transcriptome studies using global approaches, especially when exploring biological systems in a critical phase of cellular physiology.
Keywords: other
Contributor(s) Kenzelmann M, Muhlemann K
Citation(s) 11124023
Submission date Jul 02, 2003
Last update date Jun 27, 2016
Contact name Marc Kenzelmann
Phone +41 31 632 3251
Fax +41 31 632 3550
Organization name Institute of Medical Microbiology, University of Bern
Department Molecular Genome Analysis
Street address Friedbuhlstrasse 51
City Bern
ZIP/Postal code 3010
Country Switzerland
Platforms (1)
GPL4 SAGE:10:NlaIII:Homo sapiens
Samples (2)
GSM1 Foreskin Fibroblasts
GSM2 HCMV-infected foreskin fibroblasts
BioProject PRJNA85223

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